Objective To explore the application outcome of the online and offline interactive teaching method in clinical anesthesiology training for undergraduates.Methods Sixty-three students in grade three majoring in anesthesiology of Wenzhou Medical University were selected as research objects and randomly divided into 2 groups from February to July in 2018.Thirty two students from class 1 were set as experimental group and 31 students from class 2 as control group.The experimental group adopted the online and offline interactive education.The control group adopted the traditional intensive teaching method.The teaching effect was evaluated by the theoretical test results of the two groups and the survey of students in the experimental group.Results The score of theoretical test of students in the experimental group (79.31±4.54) is higher than that of students in the control group (75.61±4.88,P<0.05).Up to 87.5%(28/32)of students in the experimental group think that the online and offline interactive teaching method is more conducive to the mastery of learning content,and 78.1%(25/32)of students in the experimental group think that the online and offline interactive teaching method can enhance the comprehension and help memorizing of key learning points;mean while 78.1%(25/32)of students in the experimental group think that the online and offline interactive teaching method can also help develop good clinical thinking ability;Up to 90.6%(29/32)of the students in the experimental group hold positive attitude towards the overall learning effect can be improved by the online and offline interactive teaching method.Conclusions The online and offline interactive teaching method can not only improve students' theoretical learning,but also help cultivate students' clinical reasoning capacity.Students has given a high score for the evaluation of the online and offline interactive teaching method,good teaching effect has been achieved.