Objective To explore the application effect of bionic mold (hereinafter referred to as bionic mold) for complicated perineum laceration in midwife training. Methods Comparison method was used. From August 2017 to August 2019, the new midwives from the East Hospital, the West Hospital and the South Hospital ofFirst Maternity and Infant Hospital, Tongji University School of Medicine carried out four simulated training sessions of complex perineum laceration, each of which lasted for 2 weeks and 8 weeks, with a total of 70 trainees. In the second and eighth week of each training, the self-designed evaluation table of perineal laceration repair skills was used to evaluate the new midwives' mastery of perineal laceration repair skills before and after the training. After the training, the new midwives were assessed with self-made questions before and after the training, and the effect of bionic mold simulation training was evaluated by the new midwives through questionnaire after the training. Results Before and after training, the suture completion time of midwives was (28.16±6.57) min vs (23.54±4.54) min, the difference was statistically significant (P<0.01); the anatomical level clarity skill score was (5.75±2.44) points vs (7.83±1.24) points, the difference was statistically significant (P<0.01); the comprehensive theory examination scores were (61.45±4.76) The score ratio was (83.60±4.31), the difference was statistically significant (P<0.05); 70 new midwives gave satisfactory evaluation on 18 items of bionic mold simulation training. Conclusions Using the bionic mold of complex perineum tear in simulation training can quickly grasp the diagnosis and identification of complex perineum tear wound, and consummate the operation skills of repair. On the basis of conforming to medical ethics, the clinical teaching quality for training new midwife is significantly improved, which is worthy of clinical promotion and application.