Objective To evaluate the application of blended teaching mode based on the outcome-based education(OBE) in internship training of emergency medicine.Methods Experimental control method was used. Totally 74 eight-year program undergraduates who participated in the internship of emergency medicine in the Third Xiangya Hospital of Central South University from 2018 to 2020 were selected. Experimental group with 39 students from Grade 2015 received the blended teaching mode based on OBE. Control group with 35 students from Grade 2014 were trained by traditional teaching model. The teaching effect was evaluated and compared between the two groups.Results The students in the experimental group showed a better performance on the clinical practice assessment [(85.85±8.93) vs. (81.69±8.27)];class participation performance[79.5% (31/39) vs. 54.3% (19/35)] and the click ratio of learning platform[79.5 % (31/39) vs.57.1% (20/35)] than the control group(all P＜0.05).Conclusions A blended teaching model based OBE have an active effect on students' clinical practice, classroom learning enthusiasm and learning initiative after class.