Objective To explore the effect of diversified assessment guide implementation of team-based learning (TBL) in the standardized training for residents in department of anesthesiology. Methods Using experimental control method. Sixty residents joined the training from August 2018 to August 2019, were randomly divided into two groups as diversified assessment guide TBL group (experimental group) and traditional TBL group (control group). Design and build a diversified assessment and rating system. According to the diversified assessment scores, the students were divided into R1, R2 and R3 grades, and the learning outcomes of two groups are compared. Results The assessment scores of resident standardized training between the R1 experimental group (94.79±4.41) and the control group (90.72±4.08), the R2 experimental group (89.22±4.51) and the control group (83.08±4.23), R3 experimental group (81.63±4.81), and the control group (75.22±4.24) are all significantly different (all P<0.05). Satisfaction between experimental group (23.18±1.23) and control group (21.22±1.12) is also significantly different (t=3.61,P<0.01). Conclusions TBL teaching method under the guidance of diversified assessment and rating improves the standardized training quality of resident in anesthesiology, and residents are generally more inclined to this training mode.