Objective To report the application of a practice teaching and assessment platform in the education of medical imaging anatomy with a multi-module and digital technology.Methods Experimental control method was used in this study. A total of 91 undergraduates of medical imaging and medical imaging technology in grade 2017 were selected as the experimental group and 90 undergraduates of medical imaging and medical imaging technology in grade 2016 were selected as the control group. The experimental group was educated with a platform (containing the digital theory teaching, practice teaching, ‘cloud’ classroom and assessment modules), while the control group used traditional teaching methods. Students' examination scores of theory, practice and routine performance were compared. and subjective satisfaction to the course by students were evaluated.Results Examination scores of theory course[undergraduates of medical imaging (80.36±8.43), undergraduates of medical imaging technology (79.10±7.91)], practice course [undergraduates of medical imaging (79.18±8.26), undergraduates of medical imaging technology (80.31±8.32)] and routine performance[undergraduates of medical imaging (83.77±5.38), undergraduates of medical imaging technology (84.41±5.85)] of the experimental group were significantly higher as, compared to the control group[undergraduates of medical imaging (77.19±7.55), (75.18±6.71), (81.75±5.57),undergraduates of medical imaging technology (74.17±9.27), (74.03±6.81), (80.73±5.50), all P<0.05]. Satisfaction rate to the course in experimental group significantly higher than that of the control group, medical imaging [(82.11±4.09)vs.(78.98±1.96)], medical imaging technology[(84.31±2.35)vs.(79.17±3.16)], all P<0.05.Conclusions The multi-module digital medical imaging anatomy practice teaching and assessment platform is helpful to cultivate students' medical imaging pattern of thought, and to develop a comprehensive capacity of medical imaging and to evaluate the learning outcomes comprehensively, so this plateform and technology package concerned have a broad application prospect.