Objective Explore the application effect of mobile learning assisted teaching model in pathology teaching by comparing with traditional teaching methods.Methods A total of 1458 students from 1 to 8 classes in the 2013 to 2015 grade five-year and seven-year clinical medical program in China Medical University were selected as the research subjects. Among them, 1~4 class students were in the experimental group and 5~8 students in the control group. The two group students were taught with the same teachers. The experimental group adopted the mobile learning teaching model based on the micro lesson teaching, while the control group adopted the traditional teaching model.Results The feedback from the experimental group showed that most of the students were given an affirmative evaluation of the traditional teaching model based on the micro class based mobile learning. The T test analysis showed that the students in the experimental group were higher than the control group in the theoretical assessment, including 2013 grade seven year (t=2.23,P=0.0263), 2014 grade five year system (t=2.46,P=0.0147) and 2015 grade seven year system (t=2.05,P=0.0414) , the difference was statistically significant.Conclusions In the teaching of pathology, using micro class as the carrier of mobile learning teaching model is better than the traditional teaching model. It is beneficial to achieve the goal of teaching and to better realize the purpose of cultivating excellent talents.