Objective To explore the feasibility and learning outcome of the whole course management teaching method in ophthalmology training of general practitioners (GPs). Methods From January to December 2018,a total of 34 GPs were trained in the ophthalmology department of the China-Japan Friendship Hospital. They participated in the whole course management. At the end of the training a written case report was required and questionnaire for all 34 GPs and 8 teachers was conducted. Statistical analysis of the results was performed. Results All 34 GPs completed the learning process. The average score of the final case report was 86.1±8.2. The group differences divided by gender [(84.9±9.9) vs (86.8±7.7),t=-0.67, P=0.507], educational background [(87.3±7.9) vs (85.5±8.4), t=0.594, P=0.557], education level [(85.7±8.9) vs (86.2±8.0), t=-1.63, P=0.871] and grade [(83.3±9.5) vs (86.0±7.4) vs (91.8±4.0), F=2.404,P=0.107] were not statistically significant. The survey showed that most of the GPs believed that the teaching arrangement was professional[100%(34/34)],the learning target was achieved [94.1%(32/34)],time schedule was reasonable [100%(34/34)]. Most of them considered that the whole course management method helped them improve the ability to communicate with patients [100%(34/34)], literature retrieval ability [97.1%(33/34)], deepened the understanding of the diseases [94.1%(32/34)], increased the interest of learning [94.1%(32/34)] and helped to cultivate clinical reasoning [100%(34/34)]. Conclusions The application of whole course management in the standardized training of ophthalmology for GPs is practical and feasible, and has achieved the expected results, which is worth further promotion.