Objective To evaluate application effect of team-based learning (TBL) combined with error correction teaching in aseptic teaching. Methods Experimental control method was used. One hundred and eighty students from “5+3” clinical medicine enrolled in 2015 in Fujian Medical University were selected and randomly divided into two groups. TBL combined with team error correction teaching method was used in the experimental group, TBL combined with traditional teaching method was used in the control group. The theoretical results, aseptic performance and clinical application of aseptic technique were compared between the two groups. The satisfaction of two groups of students was surveyed by self-complied questionnaires. Results The differences between two groups in theory scores(84.5±5.9 vs 78.5±9.4, t=6.13, P<0.01), hand washing(90.8±2.7 vs 87.1±1.6, t=8.83, P<0.01), wearing clothes and gloves (92.2±3.0 vs 86.4±2.3, t=11.45, P<0.01), sterilizing and operating drape (92.0±2.9 vs 87.4±1.4, t=10.53, P<0.01), test of aseptic skill in appendectomy(9.2±0.8 vs 8.7±0.7, t=2.01, P<0.05) all have statistical meanings. The results of questionnaires showed that, in enhancing self-learning ability and team cooperation, experimental group had better recognition than that of the control group. Conclusions TBL combined with error correction teaching method can improve the theoretical level and clinical performance, to a certain extent. The students' satisfaction toward TBL combined with error correction is high, and it worth to use more in future aseptic teaching.