Objective To collect the nurses' evaluation to the professional ethics, communicating and cooperative capacity of residents from different sources in a residency institution, and to provide reference for achieving a quality homogenization. Methods Questionnaire survey was used. From April 2018 to April 2019, assessment of the professional ethics and communicating and cooperative capacity for 329 residents were conducted by 96 administrative nurses with “Digital Residence Training” APP in a residency institution. Results The proportions of the 3 indicators, “be compassionate and respect reasonable requirements of patients and their families” “be willing to listen and answer patient's questions using easy language” “dress in line with professional standards and neatly”, reflecting the professional ethics of these residents, ranked as “excellent” were 79.2%(889/1 122), 78.0%(875/1 122), and 83.9%(941/1 122), while the proportions of the 5 indicators, “respect nurse team and communicate effectively” “medical orders and medical documents are clear” “can be found when needed, respond timely to nurses report about patient's condition” “seek help timely from superior doctors and colleagues when needed” “inform nurses about the treatment and discharge plan of patients in time”, reflecting communicating and cooperative capacity, rated as “excellent” were 78.2%(877/1 122), 71.7%(804/1 122), 73.9%(829/1 122), 77.9%(874/1 122), and 71.7%(805/1 122) respectively. There was no statistically significantin scores of the professional ethics and communicating and cooperative capacity among the residents from other hospitals and graduate student, eight-year medical students and staff (P>0.05). Conclusions The professional ethics, communicating and cooperative capacity of residents were better as a whole. The professional ethics and communicating and cooperative capacity, which were homogeneous among residency from different sources.