Objective Analyzing the physical examination test results in step 1 medical licensing examination empirical research of physician, to provide new reference to improve medical licensing examination empirical research of physician. Methods The data related to the physical examination test of step 1 medical licensing examination empirical research from 2015 to 2017 were collected and analyzed, the data of 2016 was emphatically analyzed. Results The level of mastery of physical examination test module in 2015, 2016 and 2017 were 75.5%, 73.0%, 76.4% respectively. The overall level of mastery was stable among 3 years. The mastery level of each test item in physical examination test in 2016 was all above 60.0%. Conclusions The design of the test in medical licensing examination empirical research of physician was reasonable, and the applicants had generally well mastery of physical examination test.