Objective To evaluate the learing outcomes of Seminar teaching method in the field of biochemistry and molecular biology. Methods Experimental control method was used. Three hundred and twenty seven students from two classes of clinical medicine major in Hebei Medical University were selected as research subjects. A group of 157 students in class 1 was used as the experimental group, which the Seminar method was used before the class and then followed by routine lectures. A group of 170 students in class 2 was used as the control group with traditional routine lectures. The topic of the lecture in two classes is the structure and function of the protein. And then the test scores of the experimental group and the control group were compared . Results The test score of experimental group was (81.6±12.3) points, and the test score of the control group was (76.2±16.2) points. The difference was statistically significant (t=-2.75,P=0.006). Conclusions The Seminar teaching model is feasible and facilitate to creat a positive, attractive and active learning environment for students to transform passive learning into active one. It can improve the quality of teaching and to be applied in more education practice.