In determining the connotation of information literacy, this paper analyzes the present situation and the demand of medical students information literacy, in Jinzhou Medical University and related colleges and universities. According to the outcome of the investigation of medical student information literacy and information literacy training of ability standard, in combination with the practical situation of medical students, medical student information literacy evaluation index system is established. According to the index system the medical student real situation of information literacy can scientifically and objectively evaluated. It could be as the basis of cultivating medical students' information literacy. Index system was implemented and achieved good results. It played a positive role for cultivating medical student information consciousness, improving medical students' information ability, setting up the concept of lifelong learning, and cultivating information morality.