Objectives To explore the effect of using evidence-based medical(EBM)methodologies in resident standardized training.Methods Senventy-six residents who participated in the resident standardied training at Fengyang Hospital of Shanxi Province were randomly divided into experimental group and control group by computer. Using traditional teaching in control group and increase the training of evidence-based medicine methodology in experimental group. After 11 months training, anonymous questionnaire survey on the teaching outcomes was conducted through the internal network platform of the hospital. Finally questionnaires were collected from the experimental group (n=37)and the control group(n=34).Results There was no statistical difference(P>0.05) between the two groups in the knowledge of EBM, their involvement experience in scientific research work, publishing scientific research papers, reading professional manuals, using of online Chinese and foreign database, etc. Compared with control group, the experimental group performed better in learning enthusiasm, learning interest, working interest, problem analysis abilities, self-learning abilities, information acquisition abilities, abilities in choosing literatures, etc,and there were statistical difference(all P<0.05). However, there was no statistical difference in the combination between theories and clinical practices and in team collaboration(P>0.05).Conclusions EBM methodologies using in resident standardized training is helpful to raise the enthusiasm and learning interest and to improve self-learning abilities, information acquisition abilities and abilities in choosing literatures, etc. However, it takes more time and energy than traditional teaching and need to be further improvement.