Objective To explore training effect of medical students with new model of “Five In One” surgical clinical skills training.Methods 310 seven-year program medical students specializing in clinical medicine in grade 2010 and 2009 as surgery residencies from Dalian Medical University the First Clinical College were randomized into the experiment group and the control group. The students from the experiment group received “Five in One” surgical clinical skills training, and those from the control group received the traditional training, and two group of students were tested byObjective structured clinical examination (OSCE) to evaluate the teaching effect of different teaching modes. In addition, to analyze acceptance of the new training model, the students from the experiment group needed to complete a feedback survey.Results After training, surgical basic skills including suture, ligation, dressing change and so on in the experiment group compared with the control group were improved more significantly, and the differences in scoring above were all statistically significant. Survey results showed that the medical students of the experiment group generally agreed that the new training model could effectively improve medical students' learning interest and initiative, raise the level of skills and improve ability of solving problem and communication with patient and colleagues.Conclusions The new model of “Five In One” surgical clinical skills training can significantly improve the ability of medical students' surgical clinical skills, be accepted widely by the medical students and has promotional value.