Objective To analyze and evaluate the effect of the integration of excellent traditional culture in clinical departments into the occupational spirit education of medical students.Methods A simple random sampling method is used to collect 94 students of 5-year program undergraduates of clinical medicine from Shanghai Jiao Tong University School of Medicine from June 2019 to December 2019, through a self-made questionnaire to investigate the student's evaluation on the education outcome of occupational spiritual education combined with outstanding traditional culture of clinical departments. The questionnaire includes two parts: the baseline situation of the participants and the implementation of occupational spirit education (including education content, teacher evaluation and learning outcome evaluation).Results The overall score of medical students on occupational spirit education in respiratory department was (46.49±1.87) of which the content of education was (18.27±0.86), the score of the teacher evaluation and teaching effect was (18.66±0.75), and (8.56±0.36), respectively. There are 78.7% (74/94) students expressed their curiosity about the development of respiratory medicine and the stories of famous doctors. There are 93.6%(90/94)students have become more clear about the goal of practicing medicine in the future by understanding the excellent traditional culture of the respiratory department, and at the same time, they have also increased their recognition of the organic combination of traditional teaching methods and modern models in the respiratory department.Conclusions The excellent traditional culture of clinical departments further enriches the connotation of medical students' occupational spiritual education and enhances occupational identity, responsibility and honor of being a medical student.