Objective To study the correlation between psychological stress and coping style of medical undergraduates participating in ophthalmic clinical practice, and to provide scientific basis for promoting the development of mental health of medical students.Methods All 105 medical undergraduates from Sun Yat-sen University who participated in the clinical practice of ophthalmology were investigated by means of general situation questionnaire, psychological stress questionnaire and simple coping style questionnaire.Results According to the description of median and quartile distance, the total stress of Undergraduates in ophthalmology clinical practice is 3.0(3.0,4.0),and the total score of psychological stress is 105.0(98.0,110.0).The difference of psychological pressure of students with different demographic characteristics is statistically significant. The scores of positive and negative coping were 1.67(1.63,1.78)and 1.28(1.26,1.36), respectively. In terms of positive coping style, the scores of female, urban and non first practice students are higher than that of male, rural and first practice students respectively[1.69(1.65,1.78)vs 1.63(1.61,1.84),1.69(1.65,1.85)vs 1.63(1.59,1.63),1.78(1.69,1.86)vs 1.63(1.60,1.64)]; in terms of negative coping style, the scores of first practice are higher than that of non first practice students [1.28(1.25,1.32)vs 1.27(1.26,1.36)]. All kinds of psychological stress were negatively correlated with the score of positive coping (all P<0.05), while social,life,self-development,job selection stress and total stress were positively correlated with the score of negative coping (all P<0.05).Conclusions Medical undergraduates bear psychological pressure in ophthalmic clinical practice, and are influenced by gender, household registration, practical experience and other factors. At the same time, the coping style of students facing pressure is relatively single. Educators should pay close attention to the psychological stress and coping style of medical undergraduates who take part in the clinical practice of ophthalmology, carry out mental health education and guidance according to the characteristics of different groups, take effective measures to reduce the psychological stress of students and promote mental health.