Objective To understand the anxiety and depression levels ofprofessional master degree(PMD) of clinical medicine in clinical medicine in a medical college, and to find the influencing factors.Methods 1 286 PMD were surveyed by SAS and SDS, which were compiled by Zung from June to August 2019.Results The SAS score of PMD was higher than that of national norm (45.91±11.36) vs(29.78±6.46),P<0.01; and the SDS score of PMD was higher than that of national norm (49.84±13.07) vs(33.46±8.55), P<0.01. Female PMD are 1.775 times more likely to suffer from anxiety and depression than male PMD (OR=1.775, 95%CI:1.250 ~ 2.520). The risk of anxiety and depression was 1.525 times (OR=1.525, 95%CI:1.007 ~ 2.329) for PMD with research pressure from supervisors. The risk of anxiety and depression was 1.614 times that of the sleep-deprived PMD (OR=1.614, 95%CI: 1.109~2.350).Conclusions The level of anxiety and depression of PMD is higher than that in national norm. Supervisor research pressure and insufficient sleep are the risk factors of anxiety and depression of for PMD. To improve the mental health of PMD, it should be paid attention to and intervene the anxiety and depression.