Objective Through investigating the mental health status of medical graduate students of all grades(hereinafter referred to as medical graduate students), and focusing on analyzing the psychological health of the third grade graduate students,we provide theoretical basis for the psychological health counseling of medical graduates who are about to graduate.Methods Using resilience scale, stressor scale and symptom Checklist-90 (SCL-90), 941 medical postgraduates from grade 2016 to grade 2018 in three tertiary hospitals affiliated to a university were investigated and analyzed.Results The mental health level of medical graduates was generally good(64.81±9.99 vs 59.97±10.46). The mental health of the third grade medical graduates was worse than that of the first and second grade medical postgraduates(61.91±7.83 vs 67.51±11.79,67.26±9.40).The most prominent mental stress of the third grade students was employment(2.60±0.67) and study(2.51±0.70).The mental status of the third grade medical graduates who were male,single or in love was higher than that of the third grade medical graduates who were female or married (65.04±9.27 vs 60.16±10.46,P=0.008; 61.52±4.93, 64.20±7.19 vs 57.82±6.97, P=0.010). The positive rate of the third grade students was 21.9%(65/297), and the positive rate of urban students was higher than that of rural students,the difference was statistically significant(23.8% vs 18.3%, P<0.01).Conclusions The mental health problems of graduating medical graduates cannot be ignored.Urban students and married students have relatively poor psychological status. The relevant departments should take targeted measures to conduct psychological counseling and preventive intervention based on influencing factors.