Objective To understand the satisfaction degree of practical training and environment of simulated pharmacy by college students in Guizhou Province, in order to inform the construction and application of simulated pharmacy. Methods A questionnaire survey was conducted in March 2023 among 653 undergraduate and junior college students from colleges and universities in Guizhou Province who had participated in the simulated pharmacy training. The self-designed questionnaire was used, and the survey Results were statistically analyzed using the rank sum test and multiple linear regression. Results The average score of students′ overall satisfaction with the training and environment of the simulated pharmacy was 4 (3, 4) points. The single class hour of the simulated pharmacy training and the inclusion of the drug management information system, the drug shelving process, the prescription dispensing process, and the drug selection and usage guidance process are not only the influencing factors of the satisfaction with the simulated pharmacy training (β values were 1.42, 1.18, 2.15, 1.93, and 2.32 respectively, all P<0.05), but also the influencing factors of the satisfaction with the simulated pharmacy environment (β values were 1.45, 1.78, 1.78, 2.16, and 2.38 respectively, all P<0.05). The satisfaction scores for single training sessions of 4 hours, 3 hours, and 2 hours were 36(30,38), 33(28,36), and 31(27,36) respectively, and there were statistical differences (P<0.05). Conclusions The students were basically satisfied with the simulated pharmacy training and its environment. The single training time was up to 4 class hours, and the simulated pharmacy was equipped with drug management information system, drug shelving, prescription dispensing and medication guidance environment, which can help to improve the students′ satisfaction.