Radiotherapists play a crucial role in the radiation therapy process for cancer patients. Radiation therapy technology is designated to train radiotherapists, while its internship training has not yet been standardized and is still in the exploratory stage. In order to cultivate the technical skills and comprehensive qualities of students majoring in radiation therapy technology, this study attempts to establish internship training program of radiation therapy technology for this specialty from the aspects of teaching objectives, teaching plan, teaching methods, effect evaluation. From May 2020 to July 2022, this study was conducted among 42 students enrolled in the three-year radiotherapy technology program at Shandong Medical College, who were undertaking their internships in the radiotherapy departments of Peking University International Hospital and Peking University Third Hospital. At the end of internship period, the interns scored (92.06±6.28) points at theoretical knowledge assessment and (91.52±7.47) points at clinical practice assessment. The supervising teachers rated the students′ understanding of new developments in radiation therapy and their research learning situation as (4.36± 0.28) points and (3.46±0.58) points, respectively. The students′ satisfaction score for the internship was (4.62 ± 0.47) points, with 95.2% (40/42) of students believing that their theoretical knowledge and clinical skills had improved, 40.5% (17/42) believing that their radiotherapy quality control abilities had improved, and 31.0% (13/42) believing that their research capabilities had improved. The implementation of internship training program helps to enhance the theoretical knowledge and clinical skills of students in the radiation therapy technology program, but there is still room for improvement in the cultivation of radiation therapy quality control abilities and research capabilities.