Objective To investigate the medical freshmen′s demand for enrollment publicity and its influence on them and to inform further optimization of medical undergraduate enrollment publicity. Methods The method of questionnaire survey was adopted. The questionnaire was designed by the researchers. From 2021 to 2023, a pre-admission survey was conducted on 2 526 undergraduate freshmen in Peking University Health Science Center, and 2 384(94.4%) actually participated in the survey. Descriptive statistics and χ² test were used to analyze the survey results. Results Among the 2 384 freshmen, 2 146(90.0%) had been exposed to the enrollment publicity of the university, and 2 125(99.0%) of them believed that they benefited from it. As for the most needed publicity content, 1 986(83.3%) freshmen chose introduction of majors, 1 660(69.6%) freshmen chose development of disciplines, and 1 631(68.4%) freshmen chose employment prospects. As for the most preferred way of publicity, 1 106(46.4%) freshmen chose the recruitment publicity video, 1 004(42.1%) freshmen chose the senior teachers to preach. Among the freshmen, 131(5.5%) had never known about the school and major, and 64.1%(84/131) were satisfied; 385(16.1%) knew about it before high school, and 91.7%(353/385) were satisfied. There were 537(22.5%) freshmen knew about it in the first year of high school with a satisfaction of 87.7%(471/537), 564(23.7%) freshmen in the second year of high school with a satisfaction of 89.4%(504/564), 767(32.2%) freshmen in the third year of high school with a satisfaction of 86.4%(663/767).There was statistical difference among the satisfaction(P<0.05). Conclusions The publicity work of medical undergraduate enrollment covers a wide area and has a positive impact on students. Universities should focus on the introduction of majors, subject development and employment that students need most, and use promotional videos, seniors preach and other forms that students like. The earlier students get to know the school and major, the higher their satisfaction in admitting the major. Universities need to carry out publicity in advance to improve the publicity effect of enrollment.