Objective To investigate the effect of gynecological laparoscopic surgery videos combined with specific cases in probation teaching. Methods 101 five-year program clinical medical students from grade 2011 of Wuhan University were selected as the research object. They were randomly assigned into experimental group and control group according to the student ID. The experimental group received a new teaching method of gynecological laparoscopic surgery videos combined with specific cases,and the control group received the traditional teaching method. The teaching effect was evaluated through questionnaire investigation and the exam result. Results According to the survey,the experimental group was better than the control group in stimulating interest in learning,improving the understanding of pelvic anatomical structures,deepening the overall understanding of disease,training clinical thinking ability. There were statistical differences (all P<0.05). There was no significant difference between these two groups in improving communication skills (P>0.05). The test score of experimental group (84.2±6.4) was higher than that of the control group (79.0±5.4). There was statistical difference (P<0.001). Conclusions The application of gynecological laparoscopic surgery videos combined with specific cases achieved good teaching effect in probation teaching.