Nursing undergraduate students are required to complete a graduation thesis during their internship period, which poses challenges to thesis guidance and management due to their internships scattered across different hospitals. An online management system has been designed by Xi′an Medical University for graduation theses of nursing undergraduates to realize online guidance, process management, quality control and archiving of thesis materials. From July 2019 to May 2020, a total of 489 nursing undergraduates who would graduate in 2020 and 270 instructors at Xi′an Medical University used this system to complete graduation thesis related work. The outcomes were as follows: 20 papers (4.1%) were rated as excellent, 468 (95.7%) as qualified, and 1 (0.2%) as unqualified. Results from interviews with 16 supervisors and 40 undergraduates indicate that both supervisors and undergraduates found the online thesis management system to be convenient, user-friendly, and not limited by time and space. Supervisors and undergraduates can carry out thesis-related tasks anytime and anywhere. Fifteen supervisors believed that online thesis guidance did not affect their daily work and found it more amenable than traditional offline guidance. Thirty-eight undergraduates (95.0%) believed that online management simplified administrative procedures, and reduced back-and-forth between hospitals and schools for guidance sign-offs or material submissions. The online management system for graduation thesis is easy to operate, efficient, and convenient, and has been recognized by teachers and students. It is conducive to undergraduates successfully completing their graduation theses.