Objective To establish a predictive model for the development of medical students' professionalism and conduct preliminary evaluation on it. Methods Questionnaires were used among 477 clinical medicine, nursing and stomatology students from grade 2015 to 2019 in Shantou University Medical College using stratified random sampling in December 2020 to understand the influencing factors of the development of medical students' professionalism, and establish the predictive model and scoring system through single factor analysis and multivariate logistic regression analysis, and conduct preliminary evaluation based on the area under the ROC curve (AUC). Results The results showed that seven factors including gender, grade, personality, peer relationship, the education mode of “Doctor's Heart”, medical social environment and clinical practice environment were the independent predictors of the development of medical students' professionalism (P<0.05),whose OR and 95%CI were 2.223[95%CI(1.335-3.704)], 4.705[95%CI(1.761-12.574)], 3.222[95%CI(1.869-5.555)], 3.117[95%CI(1.867-5.202)], 3.840[95%CI(2.276-6.480)], 0.479[95%CI(0.282-0.816)] and 2.136[95%CI(1.257-3.629)] respectively. The AUC of ROC predicted by the model were 0.871[95%CI(0.839-0.903)]. The score of the scoring system ranged from -1 to 10, whose AUC was 0.858 [95%CI(0.824-0.891)]. With 5 points as the best truncation value of the scoring system, scores larger than 5 predicted the development trend of medical students' professionalism positively, or predicted it negatively. Conclusions The predictive model for the development of medical students' professionalism has been established, which has certain reference value in the practice of cultivating medical students' professionalism.