In order to fill the gap between training needs and short training time in the offline teaching of periodontology, the teaching and research group of periodontology at Nanjing Medical University has developed an onlineself-directed learning platform. It has been one year since the implementation of this online platform,in order to evaluate its effect, 97 undergraduates majoring in stomatology from grade 2017 in Nanjing Medical University were enrolled for the research survey. This paper expounds the design and implementation of the periodontology online self-directed learning platform, and the application effect was evaluated by final examination scores, questionnaire surveys and interviews. The results showed that average final examination score of students from periodontology rotation was (78.64±10.14) points, the excellent rate was 50% (48/96), and the pass rate was 92.71%(89/96). The overall satisfaction of the students with online self-directed learning platform was 90.7% (88/97). Therefore, the online self-learning platform helps students to master theoretical knowledge systematically and is widely acknowledged by students. In addition, students think that the platform is efficient and practical, real-time interactive, and full of challenges.