Objective To investigate and analyze the cultivation quality of 2018-2020 clinical medicine graduates of Tongji University School of Medicine and to provide reference for education reformation. Methods In March 2021, clinical medicine undergraduates graduated in the period of 2018 to 2020 from Tongji University School of Medicine were selected to be evaluated for cultivation quality. According to the training quality evaluation index system of clinical medicine graduates,questionnaire was designed to investigate the training quality of graduates based on their performance in employer institutions. The satisfaction score and demand score collected from the employers were analyzed by paired t-test. Results The results showed that 93.7% (296/316) of the graduates were satisfied with the overall status of career development and 81.4% (127/156) of employer institutions were very satisfied and 17.3% (27/156) were satisfied. In the comparison between the scores of employers on their degree of satisfaction and the scores of demand by graduates,it was shown that the employer's demand for the employees was higher than that employees meet as shown by satisfaction score:the dimension of professional quality covered two items as “having a sense of responsibility and active working attitude” [(4.86±0.53) vs. (4.81±0.46), P=0.038], “good psychological quality” [(4.81±0.55) vs. (4.69±0.64), P =0.021],the dimension of theoretical knowledge covered one item as “mastering solid basic medical knowledge” [(4.76±0.60) vs. (4.73±0.51), P=0.034],the working capacity dimension covered one item as “having the capacity of research and innovation” [(4.72±0.66) vs. (4.68±0.58), P=0.043].Conclusions Graduates were generally satisfied with the current situation of personal career development and also, employers were generally satisfied with the performance of graduates. Medical schools need to strengthen the education on students' psychological quality and the cultivation of humanistic spirit, so as to improve students' professional competence. The schools also need to promote curriculum reformation concerning theoretical knowledge teaching and further consolidate students' professional foundation and to pay more attention to the building cultivation of students' research, innovation and to improve students' professional competence.