Objective To formulate the regulation guiding the content for standardized resident training in gastroenterology. Methods The regulation guiding the content for the standardized resident training in gastroenterology was formulated based on The Gastroenterology Core Curriculum (3rd edition) through Delphi expert consultation. Results The positive coefficient of experts was 100%, and authority coefficient (CR) was 0.91. The coordination degree for the first round of expert advice was W=0.281 and that for the second round of expert advice was W=0.397. The final version of regulations cover 14 categories, i.e., “peptic ulcer disease”, “biliary tract disease and pancreatic disease”, “endoscopy”, “liver disease”, “inflammation and intestinal infectious diseases”, “malignancy”, “sports and functional disorder”, “nutrition”, “pathology”, “radiation”, “operation”, “digestive system diseases in children”, “digestive system diseases of female” and “digestive system diseases of elderly people” and 129 rules for training concerning knowledge level ranked as 36 indicators for “proficient”, 80 indicators for ”familiar” and 13 indicators for “understanding”. Conclusions The regulation used for standardized resident training in gastroenterology has been formulated which may function as a good reference to guide the training of competent gastroenterologists in China.