Objective To explore the core curriculum for medical graduates for professional degree. Methods From July 2018 to July 2021,questionnaire survey was used to investigate the current situation and demand of the curriculum by medical graduates for professional degree. The survey covered 1 281 medical graduates and 489 tutors at 48 medical colleges.The results were analyzed by Friedman rank sum test and Mann Whitney U rank sum test. Results The methodology courses and basic courses were the most abundant. There was no gap between the professional compulsory courses of postgraduates with master's degree and doctoral degree. The top three scores of the tutors on the importance of the course were the theoretical knowledge of clinical medicine [8.0(2.0)], clinical operation skills [7.0(2.0)] and professionalism and humanistic quality [7.0(6.0)](P＜0.001),the top three postgraduates' scores on the importance of the course were theoretical knowledge of clinical medicine [9.0(2.0)], clinical operation skills [9.0(2.0)] and basic medical knowledge [7.0(3.0)](P＜0.001).The scores of the importance of clinical research methodology courses and medical statistics courses of doctoral students were higher than those of master students [7.0(4.0) vs. 6.0(3.0), P=0.001; 7.0(3.0) vs. 6.0(3.0), P<0.001]. Conclusions Clinical professional theoretical knowledge, clinical operation skills, professionalism and humanistic quality, clinical research methodology and medical statistics are core course contents. The curriculum for graduates of doctor degree should be differentiated from that of master's degree. The training of research methodology and medical statistics should be intensified.