Objective The purpose of this study was to build a quality evaluation index system for clinical medicine undergraduates, and to provide a basis for the development of quality assessment and tracking investigation work for clinical medical profession. Methods The literature research method and the Delphi method were used to formulate the evaluation index of graduate training quality in clinical medicine undergraduates, and two rounds of consultation in 18 medical experts were performed to complete the determination of the indicator. The fuzzy hierarchical analysis method was used to determine the index weights. Results The recovery of the questionnaire was 100.0%,which means that the experts have high enthusiasm. The authority coefficient of experts was 0.88, which means good reliability. The variation coefficient was less than 0.25, and the Kendall's coefficient of concordance(W) was 0.246 (P<0.001), which shows the good concordance degree of expert opinions. This study constructed a comprehensive ability evaluation index system for undergraduates majoring in clinical medicine, including three primary level indicators,nine secondary level indicators and twenty-six tertiary level indicators. Conclusions This study constructed a comprehensive ability evaluation index system for undergraduates majoring in clinical medicine, including three primary level indicators of professional quality, knowledge level, and work ability, nine secondary level indicators and twenty-six tertiary level indicators, which covered the basic requirements of clinical medical undergraduates' training objective, and provided a basis for the development of quality assessment and tracking investigation work for clinical medical profession.