Objective To evaluates the teaching effect of blended teaching mode based on Canvas platform in evidence-based medicine course for postgraduates. Methods Totally 29 postgraduates who had completed learning of evidence-based medicine were enrolled as research objects.The blended method was implemented by traditional offline teaching supplemented with Canvas platform supported online teaching.At the beginning of the first class and the end of the last class, the same questionnaire was used to investigate students' understanding of knowledge of course, number of literature readed, capacity of retrieving and reading literature. Rank-sum test is used for rank variables, and χ² test is used for binary variables. Results After the course, the percentage of students who had better understanding of 23 knowledge points were significantly increased such as key assumptions of network meta-analysis from 24.1%(7/29) to 89.7%(26/29) and definition of network relationship diagram from 3.4％(1/29) to 89.7%(26/29).But the number of students who read more than 20 medical literatures per month was still 10. The number of students who could skillfully use literature retrieval tools increased from 17 to 21. There was no statistically significant difference in the capacity of literature reading and literature retrieval (all P≥0.05). Conclusions The blended teaching mode based on canvas platform has achieved good result in improving the understanding of relevant knowledge of evidence-based medicine courses for postgraduates, while the changes in literature reading quantity, literature retrieval and reading ability of students were not significant.