Objective To evaluate in situ simulation and instructive feedback teaching method in the training of supervisor of teaching process for residential training. Methods From June to August 2020, 18 trainees were selected for lecture supervisor training for in situ simulation, instructive feedback and lecturing. The training process included theoretical teaching, two rounds of skill training (demonstration-rating-feedback), offline practice and the third round of practical training (demonstration-rating-feedback). Results The median and quartile of score difference of the three rounds was 12.00(5.50, 17.00) (the first round), 4.00(2.00, 8.00) (the second round) and 6.00(2.75, 11.25) (the third round). Through Wilcoxon Matched-Pairs Signed-Ranks Test to compare with the score difference of the first round, the score difference of the second round (Z=2.49,P=0.01), there is no significant difference between the second and the third round (Z=0.78,P=0.44). Conclusions The method of in situ simulation integrated with instructive feedback effectively minimizes the difference of evaluation results (scores) from supervision experts and that from training consultant, so as to improve the consistency and accuracy of the evaluation results from the lecture supervisors.