Objective To explore the teaching effect of Flipped classroom in the fundamentals of computer of medical college.Methods A total of 209 students from the Clinical Medical College of Tianjin Medical University were studied in 2016.In the course of fundamentals of computer teaching, the experiment control method(MOOC) was adopted, and 104 students of optometry and ophthalmology were enrolled in the experimental group, and Flipped classroom teaching based on massive open online courses(MOOC) was adopted. The 105 students of pharmacy were the control group, and the traditional teaching method was adopted. At the end of the course, the final examination results and the quantitative value of curriculum participation of the two groups were compared, and the students in the experimental group were asked to conduct a questionnaire survey on Flipped classroom teaching.Results The average scores of the two groups were compared in the final examination, and the difference was statistically significant(Z=2.95,P<0.05).The quantitative comparison of curriculum participation was statistically significant(Z=25.00,P<0.05). The questionnaire results show that most of the students in the experimental group support the use of flipped classroom teaching method.Conclusions The Flipped classroom teaching based on MOOC plays an important role in cultivating students’ autonomous learning, expression induction, teamwork and logical thinking.