Objective To explore the more scientific and effective methods of medical function experiment course through investigating and analyzing the requirements and suggestions from students of different specialties. Methods A questionnaire was designed to investigate requirements and suggestions from 925 undergraduate students in many items covering their experience before, during and after the training course of medical function experiment. Chi-square tests were applied to statistical analysis of each option. Results Concerning the expectation of training time length, the proportions of students who chose 21 to 30 minutes were 43.6%(199/456) and 39.1%(75/192) by students of clinical medicine and of basic medicine respectively , which were higher than students of preventive medicine [20.9%(14/67)]. Meanwhile, the results showed that 92.7%(178/192) basic medicine students would prefer teachers to explain more detailed and profound experimental principles and this percentage was higher than that of other students. During animal experiments, more students of clinical medicine [88.8%(405/456)] and of basic medicine 88.0%(169/192) were willing to deal with potential problems by themselves under teacher's instructions than students of preventive medicine [70.2%(47/67)]. More clinical and basic medicine students favored an independent learning manner than students from other majors, the scores were(1.48±1.60)and(1.56±1.74)respectively, which were higher than preventive medicine (0.97±1.27)(all P<0.0083). Conclusions Based on the feedback, it is necessary for us to flexibly adjust training strategy for creating a specific and smart system to teach experiment course and improving the training outcomes.