As a basic compulsory course for medical students,biochemistry and molecular biology (abbreviated as biochemistry) has a direct and far-reaching influence on the construction of knowledge structure and the students' capacity building. Considering the weakness of traditional teaching of biochemistry in meeting the needs of discipline and comprehensive capacity training of students, it is an urgent demand to construct an innovative biochemistry characteristic course that meets the professional needs and conforms to the development of the times. The School of Basic Medical Sciences of Peking University has carried out in-depth teaching reform on biochemistry course and first set up a biochemistry online course that was specifically tailored for students majoring in pharmacy in 2020. Following formulating the teaching objectives according to the professional needs, the teaching content was designed accurately and the student-oriented blended teaching mode was used to stimulate students' interest in learning, to improve their understanding and application of abstract knowledge, and to build their capacity of problem-solving and self-regulated learning. Meanwhile, the ideological education was penetrated into the curriculum.